Fig. 5. The LCE-induced activation of AMPK and Sirt1. (A) Immunoblot analysis for LCE-induced AMPK phosphorylation. Levels of AMPK phosphorylation were determined in cell lysates incubated with 10 μM LCE for 30 min to 12 hr. (B) Immunoblot analysis for LCE-induced Sirt1 expression. Results were confirmed in 3 replicate experiments. (C) Schematic diagram illustrating the mechanism by which LCA and LCE inhibit LXRα-mediated SREBP-1c expression and lipogenic gene expression via the AMPK/Sirt1 pathway.
