Table 1.

N-terminal sequences of human P450 2J2 constructs for heterologous expression in E. coli

P450 2J2 constructs	N-terminal amino acids sequences	DNA sequences of forward PCR primers*
2J2-native	MLAAMGSLAAALWAVVHPRTLLLGTVAFLLAADFLKRRRPKNYPPGP	5'-AAACAGGATCCATCGATGCTTAGGAGGTCATatgctcgcggcgatggg-3'
2J2-M	MALLLAVF - - - - - - - - - - - - LLLGTVAFLLAADFLKRRRPKNYPPGP	5'-CGAATCATatggctctgttattagcagtttttctcctactgggcactgtcgccttt-3'
2J2-D	MA - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - KRRRPKNYPPGP	5'-CGAATCATatggctaaacgtcgtcgcccaaagaactacccg-3'

*DNA sequences for reverse PCR primer: 5'-TGCGCTGTTCCTCAGGTGCATCACCATCACCATCACTAAAAGCTTCCGGTCTAGAAATAAT-3'.