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Expression, distribution, and purification levels of P450 2J2 constructs
| P450 2J2 constructs | Whole cell level (nmol/liter culture) | After ultracentrifugation (nmol/L culture) | After Ni2+-NTA column (nmol/L culture) | |
|---|---|---|---|---|
|
| ||||
| Cytosolic fraction | Membrane fraction | |||
|
| ||||
| 2J2-native | ND* | ND* | ND* | ND* |
| 2J2-M | 125 | ND* | 18.6 | 8.3 |
| 2J2-D | 320 | 136 | 16 | 14/6.8** |
*Not detected, **from cytosolic fraction/from membrane fraction.
