Quantitative RT-PCR for (A) CC16, (B) SP-A, (C) SCGB3A2, (D) HSD11β1 and (E) PLUNC (n = 4 for each group; **p < 0.01 and *p < 0.05). (F) Representative immunohistochemistry for CC16 in the lung grafts before transplantation. Western blots for (G) CC16, (H) PLUNC, (I) C/EBPα, (J) C/EBP β, and β-actin on protein extracts from lung grafts taken after 3 h of mechanical ventilation and 4 h cold storage. The images are representative of 3 independent experiments.