Table 1.
The top 25 genes upregulated by hydrogen pretreatment of lung grafts and other interesting genes regulated by hydrogen treatment.
| Rank | Gene name | Accession number |
J5 score |
|---|---|---|---|
| 1a | Secretoglobin family 1A member 1 (uteroglobin) (Scgb1a1) | NM_013051.1 | 112.181 |
| 2 | P:myosin light polypeptide 7 regulatory (Myl7_) | XM_214074.4 | 82.586 |
| 3 | Collagen type I alpha 2 (Col1a2) | NM_053356.1 | 58.645 |
| 4a | Surfactant pulmonary-associated protein A1 (Sftpa1) | NM_017329.1 | 58.545 |
| 5 | Hemoglobin alpha adult chain 2 (Hba-a2) | NM_013096.1 | 57.481 |
| 6 | Niemann-Pick disease type C2 (Npc2) | NM_173118.1 | 54.093 |
| 7 | Peptidylprolyl isomerase A (cyclophilin A) (Ppia) | NM_017101.1 | 53.375 |
| 8 | S100 calcium binding protein A11 (calizzarin) (S100a11) | NM_001004095.1 | 52.942 |
| 9 | Myosin heavy polypeptide 6 cardiac muscle alpha (Myh6) | NM_017239.1 | 51.154 |
| 10 | P:RGD1561181 (RGD1561181_) | XM_001058739.1 | 50.147 |
| 11 | P:ribosomal protein L23a transcript variant 2 (Rpl23a) | XM_001060002.1 | 49.884 |
| 12 | P:actin gamma cytoplasmic (Actg_) | XM_213540.3 | 48.852 |
| 13 | P:similar to RIKEN cDNA 2410116I05 (LOC363377) | XM_343712.2 | 48.235 |
| 14a | Dual specificity phosphatase 1 (Dusp1) | NM_053769.2 | 47.277 |
| 15 | Ribosomal protein S4 X-linked (Rps4×) | NM_001007600.1 | 46.703 |
| 16 | Lysosomal-associated protein transmembrane 4A (Laptm4a) | NM_199384.1 | 46.299 |
| 17 | Insulin-like growth factor binding protein 7 (Igfbp7) | NM_001013048.1 | 45.933 |
| 18 | Keratin 19 (Krt19) | NM_199498.1 | 44.734 |
| 19 | S100 calcium binding protein A6 (S100a6) | NM_053485.2 | 43.624 |
| 20 | Ribosomal protein L32 (Rpl32) | NM_013226.2 | 43.613 |
| 21 | Tumor protein translationally-controlled 1 (Tpt1) | NM_053867.1 | 43.531 |
| 22 | Defensin beta 4 (Defb4) | NM_022544.2 | 41.889 |
| 23 | Calmodulin 2 (Calm2) | NM_017326.1 | 41.262 |
| 24 | Hemoglobin beta chain complex (Hbb) | NM_033234.1 | 40.688 |
| 25 | ATPase Ca++ transporting cardiac muscle slow twitch 2 (Atp2a2)_GI_8392934-S | NM_017290.1 | 40.471 |
| Other surfactant-related genes | |||
| 27a | Secretoglobin family 3A member 2 (Scgb3a2) | NM_001024283.1 | 39.612 |
| 50a | Hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1) | NM_017080.2 | 31.318 |
| 68 | Secretoglobin family 3A member 1 (Scgb3a1)_GI_61557201-S | NM_001013180.1 | 24.925 |
| −1a,b | Palate lung and nasal epithelium associated (Plunc)_GI_62645787-A | NM_172031.1 | −324.0 |
| ATP synthase subunits | |||
| 32a | ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide (Atp5b) nuclear gene encoding mitochondrial protein | NM_134364.1 | 37.291 |
| 74a | ATP synthase H+ transporting mitochondrial F1 complex O subunit (Atp5o) nuclear gene encoding mitochondrial protein_GI_20302060 | NM_138883.1 | 23.893 |
| 157a | ATP synthase H+ transporting mitochondrial F0 complex subunit F6 (Atp5j) nuclear gene encoding mitochondrial protein_GI_16758387-S | NM_053602.1 | 15.428 |
| 165a | ATP synthase H+ transporting mitochondrial F0 complex subunit G (Atp5 l) nuclear gene encoding mitochondrial protein_GI_47058993-S | NM_212516.1 | 15.18 |
| Stress-response gene | |||
| 66a | Heat shock protein 5 (Hspa5)_GI_25742762-S | NM_013083.1 | 25.314 |
a
Indicates genes chosen for further analysis.
b
Downregulated by hydrogen treatment.