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. 2014 May 2;4:4877. doi: 10.1038/srep04877

Figure 6. Fluorescent visualization of viral focus formation using BTP3-Neu5Ac.

Figure 6

MDCK cells were infected with influenza A virus strain A/WSN/1933 (H1N1) (a) or A/Shizuoka/833/2009 (H1N1pdm) (b). The infected cells were overlaid with a serum-free medium (SFM) containing 0.8% agarose and 2 μg/ml acetylated trypsin. After culture for 2 days (a) or 3 days (b), 100 μl of 200 μM BTP3-Neu5Ac was dropped onto the overlaid agarose-containing medium. After incubation at 37°C for 15 min, the plate was observed under UV irradiation at 354 nm.