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. 2014 May 2;4:4877. doi: 10.1038/srep04877

Figure 7. Histochemical visualization of virus-infected tissue in the mouse lung by using the BTP3-Neu5Ac assay.

Figure 7

Mice were intranasally infected with influenza A virus strain A/PR/8/1934 (H1N1). After fixation with 4% paraformaldehyde-PBS, lung sections at 1 day postinfection were incubated with 20 μM BTP3-Neu5Ac at 37°C for 20 min. Mock lung sections were from mice intranasally inoculated with PBS only. Scale bars indicate 200 μm. The lung sections were incubated with 20 μM BTP3-Neu5Ac in the presence of 1 μM zanamivir to confirm specificity of the BTP3-Neu5Ac assay for influenza A virus sialidase. Scale bars indicate 200 μm.