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. Author manuscript; available in PMC: 2014 May 2.
Published in final edited form as: Curr Biol. 2012 Mar 29;22(8):720–726. doi: 10.1016/j.cub.2012.02.070

Figure 1. Increased expression of cdt2 is necessary but not sufficient to induce Spd1 proteolysis.

Figure 1

(A) Cdt2-TAP levels in exponentially growing (log) or mitotically-arrested wild type cells (2710) and in exponentially growing yox1Δ mutant cells (2698). (B) Western blot analysis showing Spd1-TAP levels after MMS addition in a wild type strain (1766) and in a rad3Δ (2644) mutant (upper panels); spd1-TAP levels after MMS treatment of yox1Δ (2711) and rad3Δ yox1Δ (2713) cells (middle panels); and Spd1-TAP levels after MMS treatment of mitotically-arrested cells that were either wild-type for checkpoint function (2678) or DNA damage checkpoint defective (rad3Δ) mutant (2677) (lower panels). (C) Spd1-TAP levels in exponentially growing (log) or mitotically-arrested wild type cells (2678). Tubulin is shown as a loading control.