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. Author manuscript; available in PMC: 2014 May 2.
Published in final edited form as: J Mol Endocrinol. 2011 Apr 28;46(3):205–216. doi: 10.1530/JME-10-0116

Figure 4.

Figure 4

SKI-II blocks MCF-7 proliferation. (A) MCF-7 cells treated with vehicle or SKI-II for 48 h were fixed using 3·7% formaldehyde in PBS permeabilized using cold methanol and incubated with anti-Ki-67 antibody (red). Cells were washed (with PBS) and DAPI (blue) nuclear stained before imaging. Three independent experiments were performed with representative pictures shown here. (B) Quantitation of Ki-67 staining was determined as a percentage of total positive cells/image. The vehicle control was set to 1 for comparison with SKI-II treatment (**P<0·001).