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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2013 Mar 4;60:282–291. doi: 10.1016/j.freeradbiomed.2013.02.029

Figure 5. Primary blast causes impairment in tight junction proteins.

Figure 5

(A) Immunofluorescent staining of tight junction proteins: Occludin, Claudin-5 and ZO-1 in intact brain microvessels of rats at different time periods post-exposure (blast with 123 kPa peak overpressure): 6 hr, 24 hr and 24 hrR (two repeated exposures of every 24 hours). (B) Western blot analysis of Occludin, Claudin-5, ZO-1 and actin in the whole brain tissue homogenates of rats at different time points after exposure to blast (123 kPa peak overpressure). (C) Graph shows the results that are expressed as ratio of occludin/claudin5/ZO-1 to that of β-actin band. Values are mean ±SEM (n = 3). Statistically significant, **p<0.01 versus control in C. Scale bar:5 μm.