Figure 3.

Effects of LA on (A) FBS- and (B) LPS-induced ERK1/2 activation in VSMCs. Cells were treated with LA for 2 h prior to FBS (5%) or LPS (100 ng/mL) stimulation for 15 min. Whole cell lysates were prepared and subsequently used for detection of p-ERK1/2 and ERK1/2 by Western blotting. The bar graphs show the ratio of densities of phosphoproteins to total proteins for the blots. n=3. ^cP<0.01 vs control, ^eP<0.05, ^fP<0.01 vs FBS or LPS. Values are presented as mean±SEM.