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. 2014 Apr 14;3(4):e97. doi: 10.1038/oncsis.2014.11

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Copyright © 2014 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Ago2 is upregulated in HCC and negatively correlated with miR-99a. (a) The correlation between relative expression of miR-99a and Ago2 mRNA in HCC tissues as determined by qRT–PCR. (b) Increased AGO2 protein as analyzed by immunohistochemistry in cancerous tissues. The patient serial number was 30 and 6, respectively. (c) Increased AGO2 protein as detected by western blotting. Expression ratio was calculated by normalizing AGO2 to total protein levels in each case. The value of relative expression of miR-99a/AGO2 protein is shown below. The patient serial number was 6, 8, 9, 11, 30, 41, 53, 68, 73, 90, 121, and 132, respectively. N, matched noncancerous control; T, HCC tissue. (d) The correlation between expression of miR-99a and AGO2 protein in 12 pairs of HCC samples mentioned above.