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. 2014 Apr 21;3(4):e98. doi: 10.1038/oncsis.2014.13

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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(a) Schematic illustration of the DREF-binding consensus sequence in the 5′-flanking region of the bsk gene. Arrowheads show the positions of primers used for the ChIP assays for two genomic regions (region 1, proximal, and region 2, distal). (b) Chromatin immunoprecipitation results. The data shown are derived from quantitative real-time PCR analysis of two genomic regions 1 and 2. Chromatin from S2 cells was immunoprecipitated with either anti-dMLF IgG or control rabbit IgG. The fold different values are for anti-dMLF IgG immunoprecipitated samples compared with the corresponding control rabbit IgG immunoprecipitated sample defined as 1.00. A sample without antibody treatment was also included as a negative control (no antibody column).