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. 2014 Mar 4;110(9):2310–2320. doi: 10.1038/bjc.2014.118

Figure 5.

Figure 5

TAp63 affects the expression of epithelial and mesenchymal markers. HT-29 and SW-620 cells were either transfected with negative control (Neg Ctrl), TAp63 or miR-133b mimics or co-transfected with TAp63-expressing vector with miR-133b inhibitor (co-TF) for 72 h. (A) E-cadherin and vimentin induction levels were detected by RT–PCR. (B) Percentage of E-cadherin and vimentin mRNA is expressed relative to GAPDH. (C) Western blots of E-cadherin and vimentin levels in HT-29- and SW-620-transfected cells. (D) Percentage of E-cadherin and vimentin protein is expressed relative to GAPDH. All of the data represent the means±s.d. of three different experiments analysed in triplicate. *P<0.05, #P>0.05.