Figure 1.

PCR plasmid templates for PA–GFP tagging. Shaded boxes represent PA–GFP and the selectable marker as indicated. Arrows inside the boxes indicate directions of transcription, while arrows outside the boxes indicate forward (F) and reverse (R) PCR primers (see Table 1). (A, B) Plasmid templates for single PA–GFP gene tagging. (C) Plasmid template for tandem PA–GFP gene tagging. Sequences to which F2, F5 and F4 primers anneal are shown for each plasmid template. Restriction sites in the sequences are indicated by italics