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Blank chromatogram run in optimised ternary gradient programme.
Isocratic run with 100% A, 0-70 min; linear gradient to 100% B, 70-75 min; isocratic run with 100% B, 75-125 min; linear gradient to 100% C, 125-130 min; isocratic run with 100% C, 130-150 min; equilibration to 100% A, 150-160 min.
