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SMase activity in rat kidney, liver and brain tissues. N-SMase (A) and A-SMase (B) activities were measured from 7-day, 21-day, 42-day, 180-day, 510-day, and 720-day-old rat kidney, liver and brain tissues. Rat tissue homogenates were incubated with [N-methyl-14C]–sphingomyelin. Lipids were extracted and radioactivity of reaction product 14C-choline phosphate counted in a liquid scintillation counter. Acidic and neutral sphingomyelinases: A-SMase & N-SMase.
