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SM synthase activity in rat kidney, liver and brain tissues. SM synthase activities were measured from 7-day, 21-day, 42-day, 180-day, 510-day, and 720-day-old rat kidney, liver and brain tissues. Rat tissue homogenates were incubated with [N-palmitoyl-1-14C]-sphingosine. Lipids were extracted and [N-palmitoyl-1-14C]-SM isolated on TLC by autoradiography and quantitated using a liquid scintillation counter.
