Figure 1.

Atorvastatin inhibited HUVECs apoptosis induced by Hcy involving ROS. (A) Cells were pre-incubated with DPI (10 μmol/L) and NAC (1 mmol/L) for 30 min before the addition of 1 mmol/L Hcy for 24 h and apoptosis cells were counted. (B) Cells were incubated at 37 °C with 10 μmol/L H₂DCF-DA for 30 min then stimulated with 1 mmol/L Hcy for 10, 20, 30 min. (C) Effects of atorvastatin, DPI and NAC on Hcy-induced ROS within HUVECs. Cells were incubated with 10 μmol/L H₂DCF-DA for 30 min at 37 °C and pre-incubated with 10 μmol/L atorvastatin, 10 μmol/L DPI and 1 mmol/L NAC for 30 min before the addition of 1 mmol/L Hcy for 30 min. (D) The representative experiment of the Hcy induced ROS production reduced by atorvastatin. The fluorescence intensity was measured with a flow cytometer. The relative fluorescence intensity of cells was calculated relative to untreated control cells. (a) negative control(without H₂DCF-DA); (b) blank control(without Hcy);(c) 1mol/L Hcy stimulation for 30 min; (d) 1 mol/L Hcy stimulation for 30 min+pre-incubated with atorvastatin for 30 min. Results are the means±SD from three independent experiments. ^bP<0.05, ^cP<0.01 vs untreated cells. ^eP<0.05, ^fP<0.01 vs 1 mmol/L Hcy-treated cells. Ator: atorvastatin; Hcy, homocysteine.