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. 2014 Apr 25;10(5):490–499. doi: 10.7150/ijbs.7495

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Gallotannin suppressed the migration and adhesion of Hep G2 cells. (A) Effect of gallotannin on the migration of Hep G2 cells by wound healing assay. Confluent cells were scratched and then treated with gallotannin in a complete medium for 24 h. The number of cells migrated into the scratched area was photographed (×100). (B) Effect of gallotannin on the adhesion to Matrigel coated plate after 20 min exposure. Attached cells were photographed (×200) after crystal violet staining (C) Quantification of attached cells. Scale bar = 100 μm. ***, p < 0.001, vs untreated control. (D) Western blotting was performed with antibody of integrin β1 in Hep G2 cells exposed to gallotannin for 24 h.