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. 2014 Apr 25;10(5):490–499. doi: 10.7150/ijbs.7495

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Silencing of E-cadherin enhanced apoptosis and sub G1 population induced by gallotannin in Hep G2 cells. One day after transfection with control vector or E-cadherin siRNA plasmid, gallotannin (4 μM) was treated in Hep G2 cells for 48 h. (A) Effect of gallotannin on E-cadherin, PARP and pro-caspase 3 in Hep G2 cells transfected by control vector or E-cadherin siRNA plasmid. (B) Effect of gallotannin on sub G1 population in Hep G2 cells transfected by control vector or E-cadherin siRNA plasmid by FACS analysis. Bar graphs showing the percentages of sub-G1 DNA contents undergoing apoptosis. (C) Effect of gallotannin on the adhesion to Matrigel coated plate in Hep G2 cells transfected by control vector or E-cadherin siRNA plasmid ***, p < 0.001, vs untreated control. ###, p < 0.001, vs gallotannin treated group.