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. 2014 Mar 13;7(5):561–569. doi: 10.1242/dmm.013813

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2 — The Q49X mutant failed to form morphological and functional gap junctions in cell pairs. Confocal fluorescent images were presented separately or superimposed on phase-contrast images of HeLa cells (A) and N2A cells (B) expressing Cx40-YFP or Q49X-YFP. Wild-type Cx40 formed gap-junction plaque-like structures (arrows), but the Q49X mutant was retained in an intracellular compartment. Scale bars: 20 μm. (C) Junctional currents (I_j) were consistently recorded in N2A cell pairs expressing Cx40, but I_j was not observed in cell pairs expressing Q49X. Bar graph summarizes G_js of N2A cell pairs expressing YFP (negative control), Cx40-YFP or Q49X-YFP. Numbers of cell pairs are indicated. Asterisks indicate a statistical significant difference (***P<0.001) from the G_j of Cx40-YFP.