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. 2014 Mar 13;7(5):561–569. doi: 10.1242/dmm.013813

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4 — Transdominant-negative effects of Q49X on Cx43. Confocal fluorescent images of live cells were superimposed on phase-contrast images of HeLa (A) and N2A (B) cell pairs expressing fluorescent-protein-tagged connexins and/or the Q49X mutant. Putative gap-junction plaques were readily seen in cells co-expressing Cx40-YFP and Cx43-RFP (arrows), but not in cell pairs expressing mutant Q49X-YFP and Cx43-RFP. Scale bars: 20 μm. (C) Dual patch-clamp recordings of N2A cell pairs co-expressing Cx40 (or Q49X) with Cx43. Cell pairs co-expressing Q49X and Cx43 showed a significantly lower amplitude of I_j (and the calculated junctional conductance G_j, ***P<0.001) compared with cell pairs co-expressing Cx40 and Cx43. Bar graph summarizes the junctional conductance for each mutant and connexin combination. Numbers of cell pairs are indicated.