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The Q49X mutant colocalized with DsRed-ER in live HeLa cells. HeLa cells were engineered to express YFP (A), Cx40-YFP (B) or Q49X-YFP (C) together with a red fluorescent marker of the ER (DsRed-ER). Note that the mutant colocalized extensively with DsRed-ER. Insets in C show another example of colocalization. Scale bars: 20 μm.
