FIGURE 1.

Gib2 shares high homology with human Gβ-like/RACK1, parasitic L. major LACK1, and S. cerevisiae Asc1 proteins and exists as monomer and dimer. A, multiple sequence alignment was made using ClustalW (v1.83). B, Gib2 is highly homologous to RACK1/LACK1/Asc1 proteins sharing, respectively, 84, 69, and 70% in amino acid sequence similarities. C, Western blotting showing that Gib2 is capable of forming dimeric complexes under normal physiological conditions. hr, time in yeast nitrogen base minimal medium after switching from yeast extract-peptone-dextrose.