FIGURE 8.

A Gib2 interactive network demonstrates that Gib2 functions as a signal-transducing adaptor protein interconnecting many pathways in C. neoformans. Respective S. cerevisiae proteins homologous to cryptococcal proteins (E-value <1e−5) we found were first identified in the genome of S. cerevisiae (Saccharomyces Genome Database). The S. cerevisiae proteins were then compiled using the STRING database v9.0 for protein-protein interactions using a STRING confidence score over 0.7 to generate an Asc1-protein interactive network (see text). Two interactive networks were generated accordingly: one for C. neoformans Gib2 (A) and another for S. cerevisiae Asc1 (B). Thick lines in A indicate that the connections were experimentally established by us, whereas thin lines indicate the connections derived from those of S. cerevisiae.