FIGURE 8.
Fe65 is recruited to ERα target gene promoters by estrogens and required for optimal recruitment of the ERα and its coactivators. A, T47D cells were transfected with siCtrl or siFe65 and treated with EtOH or 10−7 m E2 for 45 min. ChIP assays were performed with the indicated antibodies. B, MCF-7 cells stably transfected with empty vector or Fe65 were treated with EtOH, 10−7 m E2, or 10−7 m E2 plus 10−7 m TAM (E2+TAM) for 45 min. ChIP assays were performed with the indicated antibodies. C, MCF-7 cells stably transfected with empty vector or Fe65 were treated with EtOH or 10−8 m E2 for 1 h. Cytoplasm and nucleus proteins were subjected to IB analyses with the indicated antibodies. Hsp60 and HDAC1 blots were included to show the separation of cytoplasmic and nuclear extracts.