FIGURE 1.

Transcription of rhd_2599, tusA, and dsrE2. A, genomic organization of the rhd_2599-tusA-dsrE2 gene cluster. Promoter and terminator sites predicted by BPROM (Softberry) (29) and TransTermHP (45), respectively, are indicated. Arrows below the genes indicate sequences amplified in PCR reactions 1–5 intended to evaluate (co)-transcription of the genes. B, agarose gel of the PCR products with the following expected sizes: 1, 330 bp; 2, 235 bp; 3, 495 bp; 4, 700 bp; 5, 860 bp. C, relative mRNA levels in A. vinosum during growth on sulfide, thiosulfate, and elemental sulfur compared with growth on malate. Results for rhd_2599 are shown as white bars, those for tusA as gray bars, and dsrE2 as black bars. mRNA levels for Alvin_0486 served as an endogenous reference (19). Concentrations of internal sulfur (○) and sulfate (♢) in the A. vinosum cultures are presented in the lower panels. Points represent means of three replicates. Error bars are too small to be visible in almost all cases.