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. 2014 Mar 18;289(18):12421–12434. doi: 10.1074/jbc.M113.530717

FIGURE 4.

FIGURE 4.

ARVCF is part of an RNP containing classical mRNAs and other RNA-binding proteins. Sucrose gradient centrifugation with lysates from HEK 293 cells was done with the addition of RNasin (A) or RNase A (B). Western blot was performed with antibodies against ARVCF, hnRNP H2, p68, SRSF1, and β-catenin. Top, 10% sucrose; bottom, 40% sucrose; L, load; P, pellet. C, immunoprecipitates using ARVCF antibody with or without the addition of RNase A were analyzed by Western blotting with antibodies against ARVCF, hnRNP H2, p68, SRSF1, hnRNP A1, PKP2, and symplekin. D–F, samples from RNA immunoprecipitations of ARVCF were analyzed by RT-PCR with primers against GAPDH, actin, or PKP2 mRNA (D) and GAPDH or actin pre-mRNA (E) and U2 snRNA or ACA44 small nucleolar RNA (F). G, immunoprecipitates using PKP2 antibody were analyzed by Western blotting with antibodies against PKP2 and p68, and corresponding samples from RNA immunoprecipitations were analyzed by RT-PCR with primers against GAPDH or PKP2 mRNA. L, load; con, control; IP, immunoprecipitation; post, supernatant after RNA immunoprecipitation.