FIGURE 4.
Effect upon insulin-stimulated Irs1 Ser(P)/Thr(P) following inhibition of insulin-stimulated Ser/Thr kinase pathways. A–J, CHOIR/Irs1 cells were pretreated with the indicated kinase inhibitors for 30 min before insulin stimulation (30 nm) for an additional 30 min. Cleared lysates were subject to immunoblotting with the indicated αpS/TmAbIrs1 to determine the effect upon Irs1 Ser/Thr phosphorylation. The bands were quantified on a Kodak Image Station 4000MM Pro, and the data were analyzed using Carestream Molecular Imaging software version 5.0. No Inhib, no inhibition; No Ins, no insulin; Ins, insulin.