FIGURE 10.

Effect of CTCF knockdown on ANG and RNASE4 transcription. A, CTCF was knocked down by lentivirus-mediated shRNA in DU-145 cells, and stable knockdown cell lines were established after selection with 1 μg/ml puromycin for 7 days. Left panel, qRT-PCR analyses of CTCF mRNA level in control and shRNA transfected cell lines. CTCF mRNA level was normalized to that of β-actin. Right panels, Western blot analyses of CTCF protein level. B, qRT-PCR analyses of the mRNA levels of ANG and RNASE4 in the three stable cells lines. C, ELISA analyses of secreted ANG protein from the stable control and CTCF knockdown cell lines. D, luciferase reporter activity of the full-length Pr-U in the above three stable cell lines. Bar graphs in all panels represent the means ± S.D. of three independent experiments.