FIGURE 2.

Characterization of human ANG and RNASE4 promoters. A, activity of Pr-U and Pr-L in driving luciferase reporter gene expression in various cell lines. Data shown are the means ± S.D. of six independent experiments. B, luciferase reporter activity of serial deletion mutants of Pr-U. The left panel is the schematic views of the deletion constructs. The bar graphs at the right are luciferase activities of these constructs normalized to pGL3-B control plasmid. Data shown are the means ± S.D. of four independent experiments. C, promoter activity of internal sections of Pr-U in luciferase reporter assay. The five fragments with positions as marked were cloned into pGL3-B, and the promoter activities in driving luciferase reporter gene expression were measured in DU145 cells. Data shown are the means ± S.D. of four independent experiments.