FIGURE 2.

Functional characterization of zfVDAC2. A, size exclusion chromatography of zfVDAC2. The dashed box represents a non-aggregated homogenous population of protein used for further characterization. B, 12% SDS-polyacrylamide gel stained with Coomassie Brilliant Blue showing a pure sample of zfVDAC2. C, electrophysiology recordings of zfVDAC2 in diphytanoyl phosphatidylcholine bilayers in 10 mm Tris·HCl (pH 7.4), 1 m KCl, 5 mm CaCl₂. Total point histograms at each applied voltage were plotted to calculate the conductance (G) of the open and closed state. mAU, milliabsorbance units.