FIGURE 1.

Chronic ER stress induces the eIF2α-P-independent inhibition of protein synthesis. A, protein synthesis was measured by [³⁵S]Met/Cys incorporation into proteins in S/S and A/A MEFs treated with Tg (400 nm) for the indicated times. Results are the mean of triplicate determinations. B, Western blot analysis of extracts from S/S and A/A cells treated with Tg for the indicated times. C, quantification of the intensities of phosphorylated S6K was calculated in samples from three independent experiments using NIH ImageJ software. The asterisks indicate p < 0.05. Error bars represent S.E.