FIGURE 2.

Chronic ER stress induces the eIF2α-P-independent inhibition of mRNA translation. A and B, S/S and A/A MEFs were treated with Tg for the indicated times, and the distribution of mRNAs in polysomes was analyzed using RT-qPCR. The dashed line indicates that fractions 8–12 are considered as polysomes. The association of specific mRNAs with polysomes was determined as a percentage of the polysome-associated mRNA signal over the total mRNA signal in each polysome profile. C, quantification of three independent experiments of polysome association for the indicated mRNAs over time of Tg treatment from S/S and A/A MEFs as depicted in A and B. The asterisks indicate p < 0.05 relative to the untreated cells. Error bars represent S.E.