Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 13;289(18):12647–12656. doi: 10.1074/jbc.M113.547034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Analysis of β-(1,3)-glucans of M. restricta by gel filtration chromatography on a Sephacryl S300 column. β-(1,3)-Glucans were isolated after periodate oxidization, reduction, and Smith hydrolysis of WSDN fraction and desalted by dialysis (cut off of 1 kDa). Neutral sugars were detected by phenolsulfuric acid assay (16). β-(1,3)-Glucans were detected by Lam-A digestion and p-hydroxybenzoic acid hydrazide reagent (25). The Sephacryl S300 column was calibrated with dextrans (T500, T70, T40, and T10), and glucose.