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Gel filtration chromatography of the WSDN fraction on a Sephacryl S300 column. A, native fraction; B, endo-β-(1,3)-glucanase (Lam A)-treated fraction; C, endo-β-(1,6)-glucanase treated fraction. Neutral sugars were detected by phenolsulfuric acid assay (16). β-(1,3)-Glucans were detected by Lam-A digestion and p-hydroxybenzoic acid hydrazide reagent (25). The Sephacryl S300 column was calibrated with dextrans (T500, T70, T40, and T10) and glucose.
