FIGURE 2.

The Gdown1 CTD blocks TFIIF but not TTF2. A, silver staining of purified recombinant Gdown1 NTD(1–89). B, silver staining of purified recombinant Gdown1 CTD(197–368). C, indicated amount of Gdown1 NTD incubated with EECs before adding in TFIIF or TTF2 and chased for 7 min. D and E, indicated amounts of Gdown1 (full-length) or Gdown1 CTD(197–368) incubated with EECs and 0.1 pmol of TFIIF (D) or 0.04 pmol of TTF2 (E) as indicated before reactions were chased for 7 min. Isolated RNA was separated on a denaturing gel, and the labeled transcripts were detected by phosphorimaging.