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. 2014 Mar 13;289(18):12680–12692. doi: 10.1074/jbc.M114.558981

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Gain-of-function analysis of C18ORF1. HaCaT cells were infected with indicated adenoviruses. Forty hours later, cells were starved for 12 h and then stimulated with 5 ng/ml of TGF-β. Using cell lysates, Western blot (WB) analyses were carried out using the indicated antibodies. Upper panel, anti-phosphorylated Smad2 antibody (PS2); 2nd panel, anti-Smad2 antibody; 3rd panel, anti-Jun B antibody; 4th panel, anti-p21 antibody; 5th panel, expression of C18ORF1α1 or C18ORF1α1(4A) using anti-V5 antibody; 6th panel, anti-GFP antibody; lower panel, anti-β-actin antibody.