FIGURE 1.
Detection of the three translation arrest products. A, schematic representation of M8:Ex1(WT) RNA. B, detection of three PtRs by SDS-PAGE. M8:Ex1(WT) RNA (50–250 fmol μl−1, as indicated) was translated in the absence (lane 1) or presence (lanes 2–7) of 1 mm AdoMet. After 30 min of translation, samples were treated with RNase A (lanes 5–7). The AdoMet-dependent PtR (∼30-kDa), the 21-kDa full-length translation product (FL), and the 10-kDa arrested peptide bands (AP) produced by the RNase A treatment are marked. PtR(a), blue circle; PtR(b), magenta circle; PtR(c), green circle. A representative result of triplicated experiments is shown.