FIGURE 10.

Effect of AIRAP silencing on HUVEC viability after bortezomib treatment. HUVECs were transiently transfected with AIRAP (si-AIRAP) or control (scrambled) siRNAs, and, after 10 h, cells were treated with 10 nm bortezomib (Btz) (+) or vehicle (−). 24 and 48 h after treatment, cell viability was determined by MTT assay. Data, expressed as a percentage of untreated control, represent the mean ± S.D. of three independent experiments (top panel). *, p < 0.05. The levels of AIRAP, HSP70, and β-actin as a control were determined by Western blot analysis in parallel samples (bottom panels).