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. 2014 Mar 13;289(18):12727–12747. doi: 10.1074/jbc.M113.529123

FIGURE 4.

FIGURE 4.

The ER lumenal Hsp40 Scj1 and the nucleotide exchange factor Lhs1 stabilize torsinA and torsinAΔE. CHX chases were performed as described before (see legend for Fig. 3). A, torsinA and torsinAΔE degradation in JEM1 SCJ1 and jem1Δ scj1Δ strains (*, p < 0.002 for JEM1 SCJ1 torsinA versus jem1Δ scj1Δ torsinA; p < 0.03 for JEM1 SCJ1 torsinAΔE versus jem1Δ scj1Δ torsinAΔE). B, torsinA and torsinAΔE degradation in jem1Δ and scj1Δ strains (*, p < 0.0005 for jem1Δ torsinA versus scj1Δ torsinA; p < 0.02 for jem1Δ torsinAΔE versus scj1Δ torsinAΔE). C, torsinA and torsinAΔE degradation in LHS1 and lhs1Δ strains (*, p < 0.04 for LHS1 torsinA versus lhs1Δ torsinA; p < 0.02 for LHS1 torsinAΔE versus lhs1Δ torsinAΔE). Representative Western blots are shown below. Experiments were performed at least twice, with≥2 independent replicates per experiment.