FIGURE 2.

uPAR ligation induces a motile phenotype in lung fibroblasts. Normal human lung fibroblasts were plated on FN and incubated with 10 nm scuPA (A and B, +uPA), 10 nm ATF (C, +uPA), or vehicle (No uPA) for 90 min at 37 °C. A, immunofluorescence micrograph of vinculin (green) and actin stress fiber-stained (phalloidin; red) cells. The arrowheads point to protrusive structures. B, quantification of motile phenotype as indicated. *, p < 0.05, compared with no uPA. C, fibroblast monolayer migration ± uPAR or GAPDH siRNA ± 10 nm ATF. *, p < 0.05 denotes loss of uPA-induced hypermotility versus GAPDH siRNA control; +, p < 0.05 denotes decrease in basal (−uPA) migration compared with no siRNA and GAPDH siRNA controls. Error bars, S.E.