TABLE 2.

Summary of kinetic and thermodynamic parameters for specific interactions

Dissociation constants (K_d values) were determined by ITC or SPR or both techniques. Values for the enthalpy (ΔH) and entropy (ΔS) changes and stoichiometry (n) were also determined for those interactions studied by ITC. Errors are from the fit.

Interaction	Kd	ΔH/kcal	ΔS/cal	n	Method
		kcal mol−1	cal mol−1 K−1
rFnBPA(189–505)/FgD	3.7 ± 0.1 μm				SPRa
rFnBPA(189–511)/FgD	1.5 ± 0.1 μm				SPRa
AF1/Fg	1.1 ± 0.1 μm	−6.1 ± 0.0	6.7	2.11	ITCb
AF1/Fn	0.359 ± 0.001 nm				SPRb
AF1/FgD	1.5 ± 0.1 μm				SPRb
AF1/NTD	0.393 ± 0.002 nm				SPRb
AF1/NTD	0.7 ± 0.2 nm	−35.8 ± 0.3	−78.1	0.89	ITCb

^a The data are shown in Fig. 3.

^b The data are shown in Fig. 6.