FIGURE 8.

KLF6 antagonizes TNFα-induced apoptosis. A, knockdown of KLF6 or p65 enhanced TNFα and Smac mimetic-induced apoptosis. HCT116 cells stably transfected with the indicated RNAi plasmids were untreated or treated with TNFα (100 μg/ml) and Smac mimetic (100 nm) for 6 or 12 h. The apoptotic cells were stained with annexin V-FITC and PI followed by flow cytometry analysis (upper panel). The flow cytometry data are also presented quantitatively (lower histograph). The graphs show mean ± S.D., n = 3. **, p < 0.01; T+M: TNFα+Smac mimetic. B, knockdown of KLF6 or p65-enhanced TNFα and Smac mimetic-induced cleavages of PARP1. Experiments were performed as in A except that cells were lysed and analyzed by immunoblots with the indicated antibodies.