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. 2014 Mar 25;289(18):12896–12907. doi: 10.1074/jbc.M114.550517

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Interaction of CHOP with GABA_B receptors is responsible for OGD-induced down-regulation of cell surface receptors. Neurons were transfected with a CHOP mutant that is not able to interact with GABA_B2 (CHOPΔLZ) and EGFP. After 24 h, neurons were subjected to OGD for 10 min, followed by a recovery period of 24 h. Subsequently, neurons were stained for cell surface GABA_B receptors (blue) and endogenous CHOP (red). CHOPΔLZ-overexpressing neurons were selected by means of EGFP expression. Cultures not subjected to OGD served as controls. Top panel, representative images. Scale bar = 5 μm. Bottom panel, quantification of cell surface GABA_B receptors. Data are means ± S.E.; 38 (control, non-transfected), 27 (OGD, non-transfected), 36 (control, transfected), and 45 (OGD, transfected) neurons from three experiments. **, p < 0.01; n.s., not significant (p > 0.05); Student's t test.