FIGURE 5.

Internalized Reelin-PD is retained in the intracellular compartments longer than Reelin-WT. Primary cerebral cortical neurons from reeler mice were incubated with control medium (A, D, G, and J), medium containing Reelin-WT (B, E, H, and K), or medium containing Reelin-PD (C, F, I, and L) for 3 h. They were further incubated with Reelin-free medium for 0 (A–F) or 15 h (G–L). A–C and G–I, cells were fixed and stained with anti-Reelin NTR AF3820 (green) without membrane permeabilization. Biotinylated cholera toxin B subunit (red) and Hoechst33342 nucleus staining (blue) were used to visualize the neuronal membrane and nucleus, respectively. D–F and J–L, cells were fixed, permeabilized, and stained with AF3820 (green) and anti-Rab11 (red). Nuclei were stained with Hoechst33342 (blue). Scale bars = 20 μm.