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Establishment of a monoclonal antibody that specifically recognizes Reelin protein not cleaved at the N-t site. The culture supernatant of HEK293T cells expressing Reelin-WT (WT, lane 1) or Reelin-PD (PD, lane 2), mouse cerebral cortical primary neurons (C.C., lane 3), and mouse cerebellar primary granule cells (Cb, lane 4) were analyzed by Western blotting (WB) with 2F3 antibody (A) and then reprobed with AF3820 antibody (B). FL, full-length.
