FIGURE 10.

Fluorescence confocal laser-scanning microscopy of primary mouse cortical neurons incubated in the presence of Aβ42 and WT apoE4 or apoE4[L28P]. Primary mouse cortical neurons were incubated with 25 ng/ml Aβ42 in the absence (control) or presence of 375 nm lipid-free WT apoE4 or apoE4[L28P] for 24 h, as indicated. Aβ immunostaining of cells was detected with the antibody 6E10, followed by an FITC-conjugated secondary antibody (a–d, green). F-actin was stained with rhodamine phalloidin (e, red). The merger of images d and e is shown in f.