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Mitochondrial digestion by during autophagy. Cultured hepatocytes were coloaded with MTG (0.5 μM) for 60 min and LTR (0.5 μM) for 20 min at 37°C in complete growth medium. On the microscope stage, complete growth medium was replaced KRH plus 1 μM glucagon, and a time series of confocal images of green MTG fluorescence and red LTR was collected. Time points were selected to illustrate the onset and completion of mitochondrial digestion by autophagy. One experiment representative of 10.
